Evaluation of Interferon-beta Protein Expression in HEK293T Cell-line transfected by recombinant construction, pBud.IFNß-1a

Expression Analysis of Interferon Beta Level in HEK293T Cells Using Real-Time PCR and Protein Tests

Background: Interferons are some kind of natural cytokines which express in response to a variety of antigens including viral RNA, bacterial products, and tumor proteins. Interferon beta is used in the treatment of autoimmune diseases such as multiple sclerosis. Moreover, this drug inhibits cellular proliferation as well as angiogenesis and as a result, helps to cure cancer. In this research, i...

Construction and Identification of Recombinant HEK293T Cell Lines Expressing Non-structural Protein 1 of Zika Virus

Background: Zika virus (ZIKV) infection has become a major public health problem all around the world. Early diagnosis of Zika infection is important for better management of the disease. Non-structural protein 1 (NS1) is a potential biomarker for ZIKV infections. The purpose of this study was to produce the ZIKV NS1 protein for establishing serological diagnostic methods for ZIKV. Methods: The...

طراحی و ساخت کانسترکت نوترکیب واجد ژن اینترفرون بتای جهش یافته در ناحیه‌ کزاک (Kozak) به منظور تشدید ترجمه

Background: Interferon beta is one of the most important members of group I interferons and is the main drug for multiple sclerosis treatment. Interferon beta has short half life and this compels patients to make frequent use of medicine. According to its clinical usage there is broad effort to improve translation level and protein production. There are several important factors which effect pr...

Expression of Recombinant 3-Beta Hydroxysteroid Dehydrogenase Protein in E. coli

evaluation of fat atrophy in patients with dermis fat graft of orbit by ct-scan

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